When patients plasma containing
WBCs is treated with Manaitleadsto DNA genesis
and Manacell formation,simultaneously within
seconds, which can be seen using inverted microscope
under white light as scattered particles of
irregular shape and Manacells which are regularly
round uniform with colors of the vibgyor the
nucleus being red and enlarged, with the surface
markers not seen due to a thickened cell wall
in color blue.
On addition of Mana to patients’
plasma the huge chains of DNA are formed with
varying sizes which is over 20 to 50 microns
in size as compared to the Manacell which is
close to 10 microns. And these chains of DNA
and Manacells when viewed at higher magnification
under white light gives an appearance of long
chains of DNA and smooth round polychromatic
Manacells. The pleats of the naked DNA chains
with no cell wall or nuclear membrane can be
seen and this is confirmed using the DNA stains
and sequencing done at later points in this
study.
DNA ALLELE MARKERS SHOWING POSITIVE FOR THE
DNA GENERATED
DNA ALLELE MARKERS SHOWING POSITIVE FOR THE
DNA GENERATED The DNA thus generated was sent to
an independent laboratory in Europe where allele
markers were used to identify it as being an
uncontaminated human DNA from the plasma of
a female patient, since the allele markers were
positive for both big and small chain DNAs there
was no denaturing of the protein.
On addition of Mana to whole blood the generation
of Manacells and DNA takes place. Which is confirmed
with DNA staining dye HOECHST 33258 that gives
blue fluorescence when excited under ultraviolet
light. Under normal green light and high magnification
once again the contour of the Manacells can
be seen to be uniformly round, since green light
was used here the vibgyor colors are not noticed.
There are more than one DNA particles in every
field of the specimen under study.
