The patients’ blood is collected
through one of the peripheral veins like the cubital
vein, using a vacutainer and needle, this already
contains EDTA, thus the blood does not clot. Then
it is centrifuged at 200G so that the RBCs can
be taken away and all we need is the top in between
layer called the buffy coat and the plasma. The
buffy coat is rich in WBCs which are the nucleated
cells of the blood that means it contains DNA
and is capable of producing proteins that the
body may need.
The chains of bioengineered DNA when observed
under the microscope at low and high magnification
respectively under white light. These particles
are DNA can be confirmed by the special DNA stains
that bring out fluorescence of the particles under
ultraviolet light.
